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OSMOSIS 15,
SPRING 1999

 

Contents

Director's Letter
Surf the SAPS Website without an Internet Connection....
Laboratory Manual of Plant Cytological Techniques
Practical biotechnology eMail discussion group for teachers
Equipment gains Millennium Product Status
Primary numbers in plants. Part 1. Amaryllis flowers.
More thoughts about mosses


Letter from the Director

Dear Colleague,

Thank you to all who responded to our request for volunteers to trial the ELISA kit. In the end we ran three training workshops for a total of 48 teachers. In addition several people offered to trial the kit even though they could not get to a workshop. The initial feedback is extremely encouraging and we are now looking at ways of producing a low cost ELISA kit. We'll keep you informed.

The low cost microcentrifuge (Osmosis 14 Autumn 1998) is now available from Middlesex University Services Ltd, (Teaching resources), Trent Park, Bramley Road, London N14 4YZ, tel. 0181 447 0342, fax. 0181 447 0340 at a cost of £48 plus p & p. Use this link to find out more about the centrifuge.

Lastly, Mary MacDonald has now left SAPS to work full time for Biogemma (formerly Nickersons BioCem) on the Cambridge Science Park. During her time with SAPS she put together, and ran, the widely praised SAPS web site at the same time as developing a very successful plant pathology workshop and the ELISA kit. We are very grateful for all that she has done for SAPS. In her place we welcome Erica Clark who has just retired as Biology Subject Officer for the University of London Examining Board.

Richard Price
Programme Director

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Surf the SAPS Website without an Internet Connection....

Can this really be possible? Well, almost.

The design of the SAPS website is deliberately simple. This makes it ideal for downloading to your own computer and running "off-line" (not connected to the Internet). This means that the SAPS website can be available on any machine. You can be certain that pupils only have access to the SAPS website and are not using the time to read the latest news on their favourite pop idol. There are no 'phone charges, no logging on, no passwords, no waiting for pages to be retrieved from the net and therefore, minimum supervision is needed. The website can be available in your own classroom, prep-room, mobile computer or library even though you do not have an Internet connection to those rooms. Get the potential? Want to know how? Then read on . . . .

You will need a piece of software to do the downloading on a machine which does have connection to the Internet. There are several suitable programs, but "Webbuddy" by Dataviz is the best one I have used. It is available for both Mac and PC. You can even download a free trial copy or upgrade your earlier version (version 2.0 is now available) on http://www.dataviz.com/webbuddy

With this software running on your computer, you can set up a download to take the page on display and to follow the links as far as you choose. It is normal to exclude all links outside the site in question (in this case, to stick with the SAPS website and to save, but not follow any links to other sites). You could choose to follow links on your chosen page to a particular number of levels deep, or to follow all the levels. It is safe to use all the levels with the SAPS website, and it should take less than half an hour to download the whole site.

You should then have a copy of the website which you can put on as many computers as you wish. Any computer can view the website so long as it has a browser (eg Netscape or Explorer). Webbuddy only needs to be installed on the machine used for downloading. You will not obtain the information in the database which is only searchable on-line. If a pupil clicks on a link outside SAPS, then the computer will attempt to make a connection to the Internet. If this is possible, the link will be established on-line, otherwise, an error message will be displayed and the browser will continue from the page with the link on it.

I have found this very satisfactory on several other sites too. You can even schedule Webbuddy to download a site regularly, at a chosen time, so you always have an up-to-date copy.

John Hewitson
Oundle School

Those interested in Plant Cell Studies will be interested in this book:

Laboratory Manual of Plant Cytological Techniques

by Kwiton Jong of the Royal Botanic Garden, Edinburgh.

It includes a selection of reliable staining schedules with recipes for stains and reagents. There are hints on recording observations and record keeping with techniques for preparing permanent slides and references to source material.

ISBN 1 872291 42 2 Softback, vi+96pp Price £10 + p&p £1.50 (UK), £4.50 (Overseas)

from: Print and Publications Section, Royal Botanic Garden, 20A Inverleith Row, Edinburgh EH3 5LR. Tel: 0131 552 7171 Fax: 0131 552 0382

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Practical biotechnology eMail discussion group for teachers

Would you like to keep in touch with teachers from Britain and around the world who share an interest in practical biotechnology?

The NCBE now maintains a free email discussion group for those in schools and colleges who teach secondary level biology (i.e. with students aged 11-19 years). In the UK this would include, for example, pre-GCSE, GCSE, Scottish Higher, GNVQ and 'A' level Biology students. Others with a professional interest in the teaching of biotechnology at this level are also welcome to subscribe. The mailing list is intended to help promote good practice, encourage development and to assist subscribers in resolving problems related to all aspects of the teaching of practical biotechnology. In particular, emphasis will be placed on the safety and curriculum constraints that apply in the United Kingdom and the European Union in general.

Although the primary focus is intended to be practical biotechnology, where appropriate related issues such as ethical concerns may also be addressed. Subscription to the list is entirely free. However, the discussion group is not intended for students. Consequently subscription requires a letter (on paper carrying the school or college letterhead) to be sent to: National Centre for Biotechnology Education, Science and Technology Centre, Earley Gate, University of Reading, Whiteknights, READING, RG6 6BZ, UK

The letter should state clearly the name of the intended subscriber together with his or her eMail address (a telephone number is also useful in case there are problems with the eMail address). Please ensure that you give the correct eMail address. Please also state whether you would like to subscribe to the 'ordinary' list (where you will receive messages throughout the day) or the 'digest' list (where you will receive just one message at about 4pm [UK time] each day, containing a compilation of that day's messages).

Once subscribed, all further commands (including access to archives of previous messages, other useful information and switching between 'ordinary' and 'digest' mode) can be performed directly by the subscriber. Full details will be sent to you once you have subscribed. Please note that your eMail address and other information will not be used for any other purpose than subscribing to this list.

If you have any comments or questions about the mailing list please contact Dean Madden


Tubes for Light Banks

If you're looking for materials for light banks, try LYCO Direct at 0800 525 980.

5-pack of 5' white fluorescent tubes costs £8.75 inc VAT. (Carriage and insurance (free on orders over £40) a further £4.28).

5' fluorescent light fittings with tubes are about £120 inc VAT for 8 singles or 4 twins.

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Equipment gains Millennium Product Status 

The NCBE gel electrophoresis equipment (using the new, 6-tooth comb) as used in the "Plant DNA Investigation Kit" and the Microcentrifuge designed by Prof John Cave at Middlesex University have both been awarded Millennium Product status by the Design Council. Both items have been short-listed for inclusion in the Dome.

(Details of price and availability of the centrifuge are in the Director's Letter.)

The Millennium Products initiative was launched by Tony Blair in September 1997, when he challenged businesses to demonstrate that Britain is the "creative powerhouse of the world".

You can find details of the equipment on the NCBE's website at: http://www.ncbe.reading.ac.uk

and details of all the Millennium Products at: http://www.millennium-products.org.uk

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Primary numbers in plants. Part 1. Amaryllis flowers.

There is a 30cm ruler on the pot

About this time of the year (Christmas-time), you can buy Amaryllis bulbs in the shops. All you have to do is water them each week and keep them warm (minimum 20oC). When the flower bud appears then you must water the soil about twice a week, and they will produce the most beautiful flowers.

 

When the flower stem starts to grow, measure its length every day and keep a record of the growth. Measure from the same place (for example put a ruler across the top of the pot) up to the tip. Once the flower has opened, move it to a cooler place because it will last longer there.

You can draw a line graph of the length of the flower stem each day. What shape of graph did you find?

How many flowers did you get on your flower head?

How many petals were there in each flower?

How many stamens in each flower?

Look at the stigma on top of the ovary. How many lobes has it? How many lobes does the ovary have?

Take one flower and cut the ovary in half (across its short axis - sideways, not longways). How many chambers in the ovary?

Are there any seeds in the ovary? Scrape out the ovary carefully. How many seeds did you find?

Do the flowers show symmetry? If so, what sort of symmetry?

You only need use one flower to answer all these questions - you can leave the others to develop fully and enjoy them.

You could try removing the stamens from some flowers and not from others. Do the flowers without stamens last longer than those left intact?

Did you find that things go in 3s or 4s or 5s with this flower? Try looking at snowdrops and narcissus - they are in the same family of flowers. Now look for other characters that members of the family share - what about the shape of the leaves?

After the flowers have died, cut the stalk to about 5cm above the bulb. The leaves may continue to grow and a second flower may appear. When the chance of frost has passed, bury the whole pot about 2.5 cm under the soil in your garden. Feed the plant regularly with liquid fertiliser and water it in dry periods. In early autumn, stop watering, dig up the pot and lie it on its side to dry off. The leaves will die off and the nutrients will pass back into the leaf bases to make a plump new bulb. Dry the bulb for 2 weeks at 18-24oC and then store for 10 weeks in a cool (about 14oC), dark and dry place in its pot. It is now ready for repotting and watering to wake it again.

Dr Mary MacDonald, Dr John Hewitson and staff at Laxton Junior School, Oundle

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More thoughts about mosses

Osmosis No 14 gave a list of ideas for investigations with mosses.

1) Many gardeners find it hard to kill moss in their lawns. Why is this? Perhaps mosses are not as sensitive to some weedkillers as are other 'weeds'. How could you investigate this in the laboratory?

Safety check - Always:

• take care when handling weedkillers (herbicides) and be sure to follow the manufacturers instructions and heed their warnings

• use a pipette filler when pipetting herbicides

• dispose of the solutions carefully after use. They can be put down the sink, but with plenty of water.

(In the next section, you need to make sure you understand the technical terms which are in bold.)

2) The capsules of mosses make beautiful objects to study. Carefully cut the stalk (seta) of some moss capsules at different stages of development and bring the capsules into the laboratory. Put some plasticine onto a microscope slide and poke the base of a capsule into the plasticine. Put the specimen under the microscope and observe it under low power with a reading lamp shining onto the specimen. This is viewing by 'reflected light' instead of the usual 'transmitted light'. The heat from the lamp also helps the next bit. With two mounted needles, you may be able to remove the cap (calyptra) from the capsule. You can sometimes do this by popping the calyptra off by poking it from opposite sides at the same time. Some calyptra can be lifted off by tugging at them with forceps. The peristome teeth should now be visible. These are responsible for allowing the spores out in the best weather for dispersal. They are most beautiful. If you breathe gently on the capsule, whilst looking down the microscope you should see the teeth move. Does your damp breath cause the teeth to close, or to open. Can you explain how this mechanism assists the dispersal of the spores from the capsule? There are often two rows of teeth - do you think each has a different job to do?

Did you know:

1. That mosses have a life-cycle which is very different from that of other land plants? The green leafy part of mosses (and liverworts) is haploid. The sperm and egg cells are produced in special organs (called antheridia and archegonia) by haploid mitosis.

2. That mosses produce sperm cells? Each sperm has two tails (flagella). Moss sperm find moss eggs by swimming through the film of water which covers mosses e.g. after heavy rain. They navigate by following a chemical given out by the female parts (archegonia) of the moss plant.

3. That a diploid zygote forms when a moss sperm cell fertilises a moss egg? This zygote develops into the stalked capsule which we looked at above.

4. That meiosis takes place in this capsule? The products of this meiosis are numerous tiny haploid spores which are dispersed by air currents when the capsule opens.

5. That each tiny haploid spore can develop into a new haploid moss (or liverwort) plant?

Richard Price and John Hewitson


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